CHARACTERISTICS OF BACTERIA ISOLATED FROM TERMES FOR THE PRODUCTION OF ETHANOL FROM SECOND-GENERATION BIOMASS

Abstract

Exploration of novel bacteria from termite guts for their ethanol productivity using biomass as a substrate. Several invertebrates eat wood. Wood borers, beetles, termites. Therefore during present study enzymes from bacterial isolates were extracted from termite guts and was used for conversion of lignocellulosic biomass samples into fermentable sugars and ethanol. Using 16S rRNA gene sequencing, two bacterial isolates were selected and identified. The Bacillus gene is shared by isolates TGB9 and TGB10. At 50°C and an ideal pH of 6.0, the isolates demonstrated increased xylan degradation activity. Stover, rice straw, and cotton stalk were among the agricultural substrates that were saccharified using xylanases from isolates TGB9 and TGB10. The maize stover produced results that showed increased levels of reducing sugars. Isolate TGB9's xylanases yielded greater yields of reducing sugar than isolate TGB10's. Additionally, xylanases from bacterial isolates and chemical pretreatment were compared. H2SO4 and NaOH were also used to agricultural substrates for this reason. In comparison to chemical pretreatments, the xylanases generated by TGB9 and TGB10 liberated a greater amount of sugar from agricultural substrates. Therefore, it may be said that bacteria found in termite guts are more effective than chemicals in hydrolyzing hemicelluloses.